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31.
32.
Spatial aspects of the interaction between the aphid host,Aphis gossypii, and its parasite,Allo-thrombium pulvinum larvae, on cotton plants were examined in seven fields in China. For both the host and the parasite, sample variance increased
with the mean according to Taylor's power law, which explained a substantial proportion (84–97%) of the variation in the data.
Although both the host and parasite showed aggregated distribution on cotton plants, the host aphids aggregated more strongly
than the parasites. The host dispersion pattern was not significantly affected by the presence of parasites. Increasing the
spatial scales of observation from individual plot to the entire field also had no significant effects on host and parasite
dispersion patterns. Parasites aggregated in cotton plants with higher aphid densities in five out of the seventeen samples.
Spatial patterns of parasitism were mostly host density-independent (71% of the samples) and sometimes inversely density-dependent
(29% of the sample). The processes underlying these patterns were discussed. 相似文献
33.
Fenglei Chen Yujing Wang Qinguang Liu Jiahui Hu Jiaqi Jin Zhiyu Ma Jinlong Zhang 《Journal of cellular physiology》2020,235(7-8):5666-5678
ER oxidoreduclin 1α (ERO1α) is an oxidase, participating in formation of secretory and membrane proteins. However, the other physiological functions ERO1α is not well known. We found that ERO1α is high in the Leydig cells of the testis. Therefore, the purposes of the current study are to explore the role of ERO1α and the possible mechanisms in regulating cell proliferation, apoptosis, and testosterone secretion of Leydig cells. ERO1α was mainly localized in Leydig cells in the adult mice testes by immunofluorescence staining. Western blot analysis showed that ERO1α was higher in Leydig cells than that in the seminiferous tubules. The effect of ERO1α on cell proliferation, apoptosis, and testosterone secretion was detected by transducing ERO1α overexpression and knockdown lentiviruses into cultured primary Leydig cells (PLCs) together with hCG exposure. Flow cytometry analysis showed that ERO1α promoted cell proliferation by increasing cell distribution at the S phase and decreasing that at the G0/G1 phase. Western bolt analysis showed that ERO1α increased CDK2 and CDK6 expression. Cell apoptosis determination found that ERO1α inhibited PLC apoptosis. Western bolt analysis showed that ERO1α increased the ratio of BCL-2/BAX, and decreased BAD and Caspase-3 expression. Enzyme-linked immunosorbent assay analysis demonstrated that ERO1α enhanced testosterone secretion. Western bolt analysis found that ERO1α increased StAR, 3β-HSD, and CYP17A1 expression. Furthermore, ERO1α could activate the PI3K/AKT/mTOR signaling pathway. In summary, these results suggest that ERO1α might play proliferation promotion and antiapoptotic roles and enhance testosterone secretion in PLC, at least partly, via activation of the PI3K/AKT/mTOR signaling pathway. 相似文献
34.
35.
A simple phosphorescence method is proposed for quercetin detection based on Al3+-amplified room-temperature phosphorescence (RTP) signals of 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnS quantum dots (QDs). The sensor was established based on some properties as follows. Al3+ can interact with carboxyl groups on the surface of MPA-capped Mn-doped ZnS QDs via chelation, which will lead to the aggregation of QDs and amplification of RTP signals, After the addition of quercetin, it can form more stable complex with Al3+ in alkaline aqueous solution and dissociate Al3+ from the surface of Mn-doped ZnS QDs, which will result in significant recovery of RTP intensity of the MPA-capped Mn-doped ZnS–Al3+ system. Under the optimized conditions, the change of RTP intensity was proportional to the concentration of quercetin in the range from 0.1 to 6.0 mg L−1, with a high correlation coefficient of 0.996 and a detection limit of 0.047 mg L−1. The proposed method is potentially suitable for detection of quercetin in real samples without complicated pretreatment. 相似文献
36.
37.
An experiment was conducted to investigate the effect of phytase transgenic corn (PTC) on intestinal microflora, and the fate of transgenic DNA and protein in the digesta and tissues of broilers. A total of 160 1-day-old Arbor Acres commercial male broilers were randomly assigned to 20 cages (8 chicks per cage) with 10 cages (replicates) for each treatment. Birds were fed with a diet containing either PTC (54.0% during 1–21 days and 61.0% during 22–42 days) or non-transgenic isogenic control corn (CC) for a duration of 42 days. There were no significant differences (P>0.05) between birds fed with the PTC diets and those fed with the CC diets in the quantities of aerobic bacteria, anaerobic bacteria, colibacillus and lactobacilli, or microbial diversities in the contents of ileum and cecum. Transgenic phyA2 DNA was not detected, but phyA2 protein was detected in the digesta of duodenum and jejunum of broilers fed with the PTC diets. Both transgenic phyA2 DNA and protein fragments were not found in the digesta of the ileum and rectum, heart, liver, kidney, and breast or thigh muscles of broilers fed with the PTC diets. It was concluded that PTC had no adverse effect on the quantity and diversity of gut microorganisms; Transgenic phyA2 DNA or protein was rapidly degraded in the intestinal tract and was not transferred to the tissues of broilers. 相似文献
38.
39.
Secondary structure analysis of adenovirus tripartite leader 总被引:8,自引:0,他引:8
RNA secondary structure analysis was performed to understand the translation function of the adenovirus tripartite leader, a 200-nucleotide 5' noncoding region found on all late viral mRNAs. The tripartite leader facilitates the translation of viral mRNAs at late but not early times after infection and eliminates the normal requirement for the eukaryotic initiation factor 4F or cap binding protein complex. Secondary structures were determined by probing 5' or 3' end-labeled tripartite leader RNAs under nondenaturing conditions with various single strand-specific nucleases, and the information was used to generate a potential model structure. The resulting structure is attractive since it may explain the unusual translation behavior conferred by the tripartite leader. We demonstrate that the first leader segment is predominantly single-stranded, a property consistent with the ability to enhance translation and provide independence from cap binding protein complex. In contrast, the remaining two leader segments form a moderately stable base-paired structure, except for a large hairpin loop. To confirm these findings, the secondary structure of the tripartite leader was also probed when it was attached to a large segment of a messenger RNA and was found to be very similar to that of the individual leader RNA. These findings suggest several possible mechanisms to account for the translation activity of the tripartite leader. 相似文献
40.
Jinguang Zhang 《Evolution and human behavior》2018,39(5):556-565
General trust is trust extended to people from outside one's immediate social network. Two studies have tested a parasite stress explanation of general trust using cross-cultural data, showing a linear negative correlation between parasite stress and trust in “most people.” However, recent studies suggest that 1) trust in most people as a measure of general trust confounds ingroup trust and outgroup trust in cross-cultural surveys and 2) parasite stress can curvilinearly correlate with variables of ingroup embeddedness and outgroup avoidance. Using data from the World Value Survey (WVS) Waves 5 and 6 (N?=?117,370 from 80 countries and geopolitical regions), we found no evidence that parasite stress—measured either as contemporary non-sexually-transmitted-disease (non-STD) stress or as historical pathogen prevalence—curvilinearly correlated with ingroup trust. However, parasite stress significantly curvilinearly correlated with outgroup trust, and the two-line test confirmed that the correlation was U-shaped. This research extends previous work on parasite stress and trust, informs the recent debate on whether parasite stress relates to outgroup avoidance, and suggests directions for developing the parasite stress theory of values and sociality. 相似文献